The replication of cells is responsible for tissue, organ, and species growth and reproduction. Here we describe the use of nuclear staining dyes in conjunction with image based analysis to assess the effects of compounds on cell cycle progression in adherent cells. With any anti-cancer chemotherapy it is necessary to determine not only the relative toxicity of the agent in normal and tumor cells, but also any effects these agents have on cell cycle progression. The effect of compounds on cellular proliferation is a key element of the ADME/ Tox drug discovery process. D., Laboratory Manager, Applications Department, BioTek Instruments, Inc., Winooski, VT This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. BioTek Announces New Cytation C10 Confocal Imaging Reader FebruBioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader.The honor was voted upon by SelectScience members across the world and celebrated at the 2021 Scientists’ Choice Awards online awards ceremony. BioTek's Cytation 7 Cell Imaging Multi-Mode Reader Named ‘Best New Drug Discovery & Development Product’ at Scientists’ Choice Awards BioTek Instruments is pleased to announce that the Cytation 7 Cell Imaging Multi-Mode Reader was awarded ‘Best New Drug Discovery & Development Product’ of 2020.Application flexibility extends to enzyme kinetics, protein stability and aggregation, and projects incorporating loop mediated isothermal amplification (LAMP) assays. Synergy Neo2 and Synergy H1 Now Available with Expanded Temperature Control Range JNow with temperature control up to 70 ✬, BioTek’s Synergy Neo2 Hybrid Multi-Mode Reader and Synergy H1 Hybrid Multi-Mode Reader aid in more assay workflows than ever before.A colored number corresponding to the type you are counting will be displayed on the image every time you click, and the corresponding counter is updated.Īdd the following macros to ImageJ/macros/StartupMacros.txt, restart ImageJ, and you will be able to change the counter type by pressing “1”, “2”, “3”, etc. Select the type you want to count, and count by clicking on the feature in the image. Note that at any time you can add types or remove them. Click initialize, now you are ready to count features. Open the Cell Counter plugin and the image/stack you want to count (if the Cell Counter plugin is already open you don’t need to open a new instance). Measure…: Measures the pixel value at each marker and displays a result window showing: Type - Slice - X coordinate - Y coordinate - Pixel Value.Export Image: Makes a copy of the counter image with the markers written on it (only the current slice is copied in case of a stack).Load Markers: Loads stored marker data from an XML file - Only available when running java 1.4 or higher.Save Markers: Exports the marker data to an XML file - Only available when running java 1.4 or higher.In case of a stack the counts per slice and the totals are displayed. Results: Shows the counter results in the ImageJ results table.When checked, the marker of the currently selected type closest to the mouse cursor will be deleted when you click. Delete mode: toggles between insert and delete mode.Initialize: Initializes the current image or stack for counting, this will create a duplicate image/stack titled “counter window” - when keep original is checked, the source image remains open.Opens off-screen on 1024 pixel wide and smaller monitors. May not work correctly after using Load Markers to load more than 8 counter types from an XML file. Please consider using the built-in Multi-Point Tool in ImageJ, as this tool now replicates most of the functionality of Cell Counter.

Imagej software cell staining